Challenges in the identification of Ochrobactrum anthropi in blood and sputum cultures of patients with cystic fibrosis

Authors

  • Carlos Emílio Levy State University of Campinas. , São Paulo, Campinas, Brazil.
  • Fernando Augusto LIma Marson State University of Campinas. , São Paulo, Campinas, Brazil. http://orcid.org/0000-0003-4955-4234
  • Élio Barreto Carvalho Filho State University of Campinas. , São Paulo, Campinas, Brazil.

DOI:

https://doi.org/10.17058/reci.v1i2.9967

Abstract

Background and objectives: The Ochrobactrum anthropic identification is a challenge. In this context, we perform numerous methods to identify the O. anthropic and associated the achieved results among the methods. Methods: We performed the follow methods: manual phenotypic, Vitek®2, Phoenix™, polymerase chain reaction, MALDI-TOF Vitek MS™ and BioTyper™. Results: Biochemical tests showed limitations to identify O. anthropic. Vitek®2 was the only method that showed the same result seen in polymerase chain reaction for O. anthropic, in all samples, followed by Phoenix™. MALDI-MS™ failed at defining at species level, and MALDI-BD™ presented high diversity in identification, showing to be a test of low specificity. Conclusions: Each method showed advantages and disadvantages to identify the O. anthropic. Keywords: MALDI-TOF; PCR; Phoenix™; Ochrobactrum anthropic; Vitek®2

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Author Biographies

Carlos Emílio Levy, State University of Campinas. , São Paulo, Campinas, Brazil.

Center of Clinical Pathology, Department of Pediatrics, School of Medical Sciences, State University of Campinas. Tessália Vieira de Camargo Street, 126, Cidade Universitária Zeferino Vaz, Barão Geraldo, São Paulo, Campinas, Brazil; ZIP CODE: 13083-887.

Fernando Augusto LIma Marson, State University of Campinas. , São Paulo, Campinas, Brazil.

Department of Pediatrics, School of Medical Sciences, State University of Campinas. Tessália Vieira de Camargo Street, 126, Cidade Universitária Zeferino Vaz, Barão Geraldo, São Paulo, Campinas, Brazil; ZIP CODE: 13083-887. Center of Research in Pediatrics, Department of Pediatrics, School of Medical Sciences, State University of Campinas. Tessália Vieira de Camargo Street, 126, Cidade Universitária Zeferino Vaz, Barão Geraldo, São Paulo, Campinas, Brazil; ZIP CODE: 13083-887. Department of Medical Genetics, School of Medical Sciences, State University of Campinas. Tessália Vieira de Camargo Street, 126, Cidade Universitária Zeferino Vaz, Barão Geraldo, São Paulo, Campinas, Brazil; ZIP CODE: 13083-887.

Élio Barreto Carvalho Filho, State University of Campinas. , São Paulo, Campinas, Brazil.

Department of Pediatrics, School of Medical Sciences, State University of Campinas. Tessália Vieira de Camargo Street, 126, Cidade Universitária Zeferino Vaz, Barão Geraldo, São Paulo, Campinas, Brazil; ZIP CODE: 13083-887

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Published

2018-04-02

How to Cite

Levy, C. E., Marson, F. A. L., & Carvalho Filho, Élio B. (2018). Challenges in the identification of Ochrobactrum anthropi in blood and sputum cultures of patients with cystic fibrosis. Revista De Epidemiologia E Controle De Infecção, 8(2), 189-191. https://doi.org/10.17058/reci.v1i2.9967

Issue

Vol. 8 No. 2 (2018)

Section

BRIEF COMMUNICATIONS

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